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Microcon Inc microcon mwco-100 concentrator
Microcon Mwco 100 Concentrator, supplied by Microcon Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microcon+mwco-100+concentrator/microcon+ym+10/pm38720088-261-42-42
Average 90 stars, based on 1 article reviews
microcon mwco-100 concentrator - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Filtration:

Article Title: Cryo-EM structure of the Rev1-Polζ holocomplex reveals the mechanism of their cooperativity in translesion DNA synthesis.
Article Snippet: Rev1–Polζ-dependent translesion synthesis (TLS) of DNA is crucial for maintaining genome integrity.. To elucidate the mechanism by which the two polymerases cooperate in TLS, we determined the cryogenic electron microscopic structure of the Saccharomyces cerevisiae Rev1–Polζ holocomplex in the act of DNA synthesis (3.53 Å).. We discovered that a composite N-helix-BRCT module in Rev1 is the keystone of Rev1–Polζ cooperativity, interacting directly with the DNA template–primer and with the Rev3 catalytic subunit of Polζ.

Article Title: Cryo-EM structure of the Rev1–Polζ holocomplex reveals the mechanism of their cooperativity in translesion DNA synthesis
Article Snippet: Fractions 8–10 from the gel filtration step (panel A) containing stoichiometric amounts of each subunit of Rev1-Polζ were pooled and incubated with 200 μl Glutathione Sepharose beads to remove any residual prescission protease and the resulting protein preparation was concentrated using a microcon MWCO-100 concentrator.

Incubation:

Article Title: Cryo-EM structure of the Rev1-Polζ holocomplex reveals the mechanism of their cooperativity in translesion DNA synthesis.
Article Snippet: Rev1–Polζ-dependent translesion synthesis (TLS) of DNA is crucial for maintaining genome integrity.. To elucidate the mechanism by which the two polymerases cooperate in TLS, we determined the cryogenic electron microscopic structure of the Saccharomyces cerevisiae Rev1–Polζ holocomplex in the act of DNA synthesis (3.53 Å).. We discovered that a composite N-helix-BRCT module in Rev1 is the keystone of Rev1–Polζ cooperativity, interacting directly with the DNA template–primer and with the Rev3 catalytic subunit of Polζ.

Article Title: Cryo-EM structure of the Rev1–Polζ holocomplex reveals the mechanism of their cooperativity in translesion DNA synthesis
Article Snippet: Fractions 8–10 from the gel filtration step (panel A) containing stoichiometric amounts of each subunit of Rev1-Polζ were pooled and incubated with 200 μl Glutathione Sepharose beads to remove any residual prescission protease and the resulting protein preparation was concentrated using a microcon MWCO-100 concentrator.



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